
THE CENTRAL DOGMA OF MOLECULAR BIOLOGY
QUESTIONS:
1. What is the correct order of information flow in bacterial and eukaryotic cells?
2. What are the function of enzymes involved in the replication process?
3. RNA grows in the _________ direction, as RNA polymerase moves along the template DNA strand in the ___________ direction?
4. What is the role of tRNA in translation process?
5. Suppose you mix the following components of protein synthesis in a test tube : amino acid from a rabbit; ribosomes from a dog; tRNAs from a mouse; mRNA from a chimpanzee; and necessary enzymes plus an energy source from a giraffe. If protein synthesis occurs, which animal's protein will be made?
Goodluck!
ANSWERS:
1. DNA---> mRNA---> protein
2. Helicase- Open the double helix at replication forks by distrupting the hydrogen bonds that hold the two strands together.
Single-Strand Binding (SSB) Protein- Binds to single strands of DNA and prevents the helix from reforming before it can be used as a template for replication.
Topoisomerase- Breaks one or both DNA strands, preventing excessive coiling during replication, and then rejoins them in a more relaxed configuration.
DNA polymerase- Links nucleotide subunits to form a new DNA strand from a DNA template.
DNA primase- Synthesizes short RNA primers on the lagging strand. Begins replication of the leading strand.
DNA ligase- Links Okazaki fragments by joining the 3' end of the new DNA fragment to the 5' end of the adjoining DNA.
3. 5'--->3' ; 3'--->5'
4. To transport amino acid to the ribosome
5. Chimpanzee